DEVELOPMENT OF THE METHOD OF OBTAINING THE XENOPERICARDIUM-BASED EXTRACELLULAR MATRIX AND ASSESSMENT OF ITS PROPERTIES

Venediktov Aleksey Aleksandrovich, Director of “Cardioplant” Ltd. (1 Tsentralnaya street, Penza, Russia), venediktovpenza@gmail.com
Fuki Valentina Konstantinovna, Candidate of chemical sciences, scientific adviser, “Cardioplant” Ltd. (1Tsentralnaya street, Penza, Russia), venediktovpenza@gmail.com
Gengin Mikhail Trofimovich, Doctor of biological sciences, professor, head of sub-department of biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), gengin07@yandex.ru
Kalmin Oleg Vital'evich, Doctor of medical sciences, professor, head of sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), ovkalmin@gmail.com
Nikishin Dmitriy Viktorovich, Candidate of medical sciences, associate professor, sub-department of human anatomy, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), nikishindv@gmail.com
Zhivaeva Lyubov' Vladimirovna, Postgraduate student, Penza State University (40 Krasnaya street, Penza,Russia), lyubasha8891@mail.ru

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Background. The article aims at development of a method of chemical- enzymatic modification of xenopericardium to produce extracellular matrix with high speed of bioresorption and replacement by natural tissues.
Materials and methods. After sampling the xenopericardium was treated by two ways. After that by the samples from each group the authors studied the biomechanical properties of biomaterials. After studying the biomechanical properties the remaining samples were implanted into experimental animals. Implantation period was 2 weeks, 1 month and 2 months. After removal of animals from the experiment the authors carried out histological examination of the samples.
Results. The authors developed a method of chemical-enzymatic treatment of xenopericardial bovine tissue to produce extracellular matrix with high rate of resorption and substitution by natural tissues. The researchers studied physical and mechanical properties of the resulting material and its behavior in model studies «in vivo».
Conclusions. Taking into account the results of the study of physical and mechanical properties and histological studies of biomaterials, the authors conclude that by changing the protocol of chemical-enzymatic treatment of xenopericardium it is possible to create biomaterial with less robust performance, but characterized by high rate of bioresorption and the process of replacement by natural tissues.

tissue engineering, bovine xenopericardium, chemical-enzymatic treatmen, boiresorption, elastic-deformative properties.

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